Improved Production of Gutted Adenovirus in Cells Expressing Adenovirus Preterminal Protein and DNA Polymerase

Production of gutted, or helper-dependent, adenovirus vectors by current methods is inefficient. Typically, a plasmid form of the gutted genome is transfected with helper viral DNA into 293 cells; the resulting lysate is serially passaged to increase the titer of gutted virions. Inefficient producti...

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Main Authors: Hartigan-O’Connor, Dennis, Amalfitano, Andrea, Chamberlain, Jeffrey S.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 1999
Teme:
Gene Therapy
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC104313/
https://ncbi.nlm.nih.gov/pubmed/10438876
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