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Characterization of Soluble Hepatitis C Virus RNA-Dependent RNA Polymerase Expressed in Escherichia coli

Production of soluble full-length nonstructural protein 5B (NS5B) of hepatitis C virus (HCV) has been shown to be problematic and requires the addition of salts, glycerol, and detergents. In an effort to improve the solubility of NS5B, the hydrophobic C terminus containing 21 amino acids was removed...

詳細記述

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書誌詳細
出版年:J Virol
主要な著者: Ferrari, Eric, Wright-Minogue, Jacquelyn, Fang, Jane W. S., Baroudy, Bahige M., Lau, Johnson Y. N., Hong, Zhi
フォーマット: Artigo
言語:Inglês
出版事項: American Society for Microbiology (ASM) 1999
主題:
オンライン・アクセス:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC103993/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9882374/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.73.2.1649-1654.1999
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