Characterization of Soluble Hepatitis C Virus RNA-Dependent RNA Polymerase Expressed in Escherichia coli

Production of soluble full-length nonstructural protein 5B (NS5B) of hepatitis C virus (HCV) has been shown to be problematic and requires the addition of salts, glycerol, and detergents. In an effort to improve the solubility of NS5B, the hydrophobic C terminus containing 21 amino acids was removed...

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Pubblicato in:	J Virol
Autori principali:	Ferrari, Eric, Wright-Minogue, Jacquelyn, Fang, Jane W. S., Baroudy, Bahige M., Lau, Johnson Y. N., Hong, Zhi
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1999
Soggetti:	Replication
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC103993/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9882374/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.73.2.1649-1654.1999
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