Characterization of Soluble Hepatitis C Virus RNA-Dependent RNA Polymerase Expressed in Escherichia coli

Production of soluble full-length nonstructural protein 5B (NS5B) of hepatitis C virus (HCV) has been shown to be problematic and requires the addition of salts, glycerol, and detergents. In an effort to improve the solubility of NS5B, the hydrophobic C terminus containing 21 amino acids was removed...

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Main Authors: Ferrari, Eric, Wright-Minogue, Jacquelyn, Fang, Jane W. S., Baroudy, Bahige M., Lau, Johnson Y. N., Hong, Zhi
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 1999
Fag:
Replication
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC103993/
https://ncbi.nlm.nih.gov/pubmed/9882374
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