AFM characterization of single strand-specific endonuclease activity on linear DNA

The specificity of nucleases for nicked and un-nicked double-stranded DNA has been characterized using atomic force microscopy (AFM). We have found that AFM has advantages over the usual macroscopic analyses, such as sucrose gradient centrifugation or electrophoresis, in characterizing nuclease dige...

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Bibliografische Detailangaben

Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Umemura, Kazuo, Nagami, Fuji, Okada, Takao, Kuroda, Reiko
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 2000
Schlagworte:	NAR Methods Online
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC103312/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10756206/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.9.e39
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