AFM characterization of single strand-specific endonuclease activity on linear DNA

The specificity of nucleases for nicked and un-nicked double-stranded DNA has been characterized using atomic force microscopy (AFM). We have found that AFM has advantages over the usual macroscopic analyses, such as sucrose gradient centrifugation or electrophoresis, in characterizing nuclease dige...

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Опубликовано в::	Nucleic Acids Res
Главные авторы:	Umemura, Kazuo, Nagami, Fuji, Okada, Takao, Kuroda, Reiko
Формат:	Artigo
Язык:	Inglês
Опубликовано:	Oxford University Press 2000
Предметы:	NAR Methods Online
Online-ссылка:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC103312/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10756206/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.9.e39
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