AFM characterization of single strand-specific endonuclease activity on linear DNA

The specificity of nucleases for nicked and un-nicked double-stranded DNA has been characterized using atomic force microscopy (AFM). We have found that AFM has advantages over the usual macroscopic analyses, such as sucrose gradient centrifugation or electrophoresis, in characterizing nuclease dige...

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Bibliografiske detaljer

Udgivet i:	Nucleic Acids Res
Principais autores:	Umemura, Kazuo, Nagami, Fuji, Okada, Takao, Kuroda, Reiko
Format:	Artigo
Sprog:	Inglês
Udgivet:	Oxford University Press 2000
Fag:	NAR Methods Online
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC103312/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10756206/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.9.e39
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