A method for quantification of absolute amounts of nucleic acids by (RT)–PCR and a new mathematical model for data analysis

Accurate quantification of nucleic acids by competitive (RT)–PCR requires a valid internal standard, a reference for data normalization and an adequate mathematical model for data analysis. We report here an effective procedure for the generation of homologous RNA internal standards and a strategy f...

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Dettagli Bibliografici

Pubblicato in:	Nucleic Acids Res
Autori principali:	Vu, Huong L., Troubetzkoy, Serge, Nguyen, Huan H., Russell, Michael W., Mestecky, Jiri
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 2000
Soggetti:	NAR Methods Online
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC102801/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10710435/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.7.e18
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