Modification of human β-globin locus PAC clones by homologous recombination in Escherichia coli

We report here modifications of human β-globin PAC clones by homologous recombination in Escherichia coli DH10B, utilising a plasmid temperature sensitive for replication, the recA gene and a wild-type copy of the rpsL gene which allows for an efficient selection for plasmid loss in this host. High...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Imam, A. M. Ali, Patrinos, George P., de Krom, Mariken, Bottardi, Stefania, Janssens, Rick J., Katsantoni, Eleni, Wai, Albert W. K., Sherratt, David J., Grosveld, Frank G.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 2000
Assuntos:	NAR Methods Online
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC102750/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10871388/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.12.e65
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