Modification of human β-globin locus PAC clones by homologous recombination in Escherichia coli

We report here modifications of human β-globin PAC clones by homologous recombination in Escherichia coli DH10B, utilising a plasmid temperature sensitive for replication, the recA gene and a wild-type copy of the rpsL gene which allows for an efficient selection for plasmid loss in this host. High...

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Bibliografiske detaljer
Main Authors: Imam, A. M. Ali, Patrinos, George P., de Krom, Mariken, Bottardi, Stefania, Janssens, Rick J., Katsantoni, Eleni, Wai, Albert W. K., Sherratt, David J., Grosveld, Frank G.
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2000
Fag:
NAR Methods Online
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC102750/
https://ncbi.nlm.nih.gov/pubmed/10871388
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