A dual reporter screening system identifies the amino acid at position 82 in Flp site-specific recombinase as a determinant for target specificity
We have developed a dual reporter screen in Escherichia coli for identifying variants of the Flp site-specific recombinase that have acquired reactivity at an altered target site (mFRT). In one reporter, the lacZα gene segment is flanked by mFRTs in direct orientation. In the other, the red fluoresc...
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| Udgivet i: | Nucleic Acids Res |
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| Principais autores: | , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
2002
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC101850/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11917027/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/30.7.1656 |
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