Magnesium is required for specific DNA binding of the CREB B-ZIP domain

We have examined binding of the CREB B-ZIP protein domain to double-stranded DNA containing a consensus CRE sequence (5′-TGACGTCA-3′), the related PAR, C/EBP and AP-1 sequences and the unrelated SP1 sequence. DNA binding was assayed in the presence or absence of MgCl(2) and/or KCl using two methods:...

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Main Authors: Moll, Jonathan R., Acharya, Asha, Gal, Jozset, Mir, Alain A., Vinson, Charles
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2002
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Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC101231/
https://ncbi.nlm.nih.gov/pubmed/11861917
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