Simultaneous detection of CpG methylation and single nucleotide polymorphism by denaturing high performance liquid chromatography

We report here a novel method to simultaneously detect CpG methylation and single nucleotide polymorphisms (SNPs) using denaturing high performance liquid chromatography (DHPLC). PCR products of bisulfite-modified CpG islands were separated using DHPLC. BstUI digestion and DNA sequencing were used i...

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Hlavní autoři: Deng, Dajun, Deng, Guoren, Smith, Michael F., Zhou, Jing, Xin, Huijun, Powell, Steven M., Lu, Youyong
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2002
Témata:
NAR Methods Online
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC100312/
https://ncbi.nlm.nih.gov/pubmed/11809901
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