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Simultaneous detection of CpG methylation and single nucleotide polymorphism by denaturing high performance liquid chromatography

We report here a novel method to simultaneously detect CpG methylation and single nucleotide polymorphisms (SNPs) using denaturing high performance liquid chromatography (DHPLC). PCR products of bisulfite-modified CpG islands were separated using DHPLC. BstUI digestion and DNA sequencing were used i...

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Dades bibliogràfiques
Publicat a:Nucleic Acids Res
Autors principals: Deng, Dajun, Deng, Guoren, Smith, Michael F., Zhou, Jing, Xin, Huijun, Powell, Steven M., Lu, Youyong
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2002
Matèries:
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC100312/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11809901/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/30.3.e13
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