Simultaneous detection of CpG methylation and single nucleotide polymorphism by denaturing high performance liquid chromatography

We report here a novel method to simultaneously detect CpG methylation and single nucleotide polymorphisms (SNPs) using denaturing high performance liquid chromatography (DHPLC). PCR products of bisulfite-modified CpG islands were separated using DHPLC. BstUI digestion and DNA sequencing were used i...

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Bibliographic Details

Published in:	Nucleic Acids Res
Main Authors:	Deng, Dajun, Deng, Guoren, Smith, Michael F., Zhou, Jing, Xin, Huijun, Powell, Steven M., Lu, Youyong
Format:	Artigo
Language:	Inglês
Published:	Oxford University Press 2002
Subjects:	NAR Methods Online
Online Access:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC100312/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11809901/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/30.3.e13
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