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Imino proton NMR analysis of HDV ribozymes: nested double pseudoknot structure and Mg(2+) ion-binding site close to the catalytic core in solution

Minimized trans-acting HDV ribozyme systems consisting of three (Rz-3) and two (Rz-2) RNA strands were prepared and their folding conformations were analyzed by NMR spectroscopy. The guanosine residues in one of the enzyme components of Rz-3 were labeled with (13)C and (15)N. Imino proton signals we...

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Detalhes bibliográficos
Main Authors: Tanaka, Yoichiro, Hori, Tamaki, Tagaya, Mitsuhiro, Sakamoto, Taiichi, Kurihara, Yasuyuki, Katahira, Masato, Uesugi, Seiichi
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2002
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC100292/
https://ncbi.nlm.nih.gov/pubmed/11809890
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