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Technical adequacy of bisulfite sequencing and pyrosequencing for detection of mitochondrial DNA methylation: Sources and avoidance of false-positive detection.

The existence of cytosine methylation in mammalian mitochondrial DNA (mtDNA) is a controversial subject. Because detection of DNA methylation depends on resistance of 5'-modified cytosines to bisulfite-catalyzed conversion to uracil, examined parameters that affect technical adequacy of mtDNA m...

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Bibliografske podrobnosti
Main Authors: Chie Owa, Matthew Poulin, Liying Yan, Toshi Shioda
Format: Artigo
Jezik:Inglês
Izdano: Public Library of Science (PLoS) 2018-01-01
Serija:PLoS ONE
Online dostop:http://europepmc.org/articles/PMC5805350?pdf=render
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