Efficient isolation of pure and functional mitochondria from mouse tissues using automated tissue disruption and enrichment with anti-TOM22 magnetic beads.

To better understand molecular mechanisms regulating changes in metabolism, as observed e.g. in diabetes or neuronal disorders, the function of mitochondria needs to be precisely determined. The usual isolation methods such as differential centrifugation result in isolates of highly variable quality...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Andras Franko, Olivier R Baris, Eva Bergschneider, Christine von Toerne, Stefanie M Hauck, Michaela Aichler, Axel K Walch, Wolfgang Wurst, Rudolf J Wiesner, Ian C D Johnston, Martin Hrabĕ de Angelis
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Public Library of Science (PLoS) 2013-01-01
Cyfres:PLoS ONE
Mynediad Ar-lein:http://europepmc.org/articles/PMC3861405?pdf=render
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