Efficient Isolation of Pure and Functional Mitochondria from Mouse Tissues Using Automated Tissue Disruption and Enrichment with Anti-TOM22 Magnetic Beads

To better understand molecular mechanisms regulating changes in metabolism, as observed e.g. in diabetes or neuronal disorders, the function of mitochondria needs to be precisely determined. The usual isolation methods such as differential centrifugation result in isolates of highly variable quality...

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Main Authors: Franko, Andras, Baris, Olivier R., Bergschneider, Eva, von Toerne, Christine, Hauck, Stefanie M., Aichler, Michaela, Walch, Axel K., Wurst, Wolfgang, Wiesner, Rudolf J., Johnston, Ian C. D., de Angelis, Martin Hrabĕ
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2013
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3861405/
https://ncbi.nlm.nih.gov/pubmed/24349272
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0082392
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