Efficient isolation of pure and functional mitochondria from mouse tissues using automated tissue disruption and enrichment with anti-TOM22 magnetic beads.

To better understand molecular mechanisms regulating changes in metabolism, as observed e.g. in diabetes or neuronal disorders, the function of mitochondria needs to be precisely determined. The usual isolation methods such as differential centrifugation result in isolates of highly variable quality...

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Egile Nagusiak: Andras Franko, Olivier R Baris, Eva Bergschneider, Christine von Toerne, Stefanie M Hauck, Michaela Aichler, Axel K Walch, Wolfgang Wurst, Rudolf J Wiesner, Ian C D Johnston, Martin Hrabĕ de Angelis
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Public Library of Science (PLoS) 2013-01-01
Saila:PLoS ONE
Sarrera elektronikoa:http://europepmc.org/articles/PMC3861405?pdf=render
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