Quantitative assessment of the sensitivity of various commercial reverse transcriptases based on armored HIV RNA.

The in-vitro reverse transcription of RNA to its complementary DNA, catalyzed by the enzyme reverse transcriptase, is the most fundamental step in the quantitative RNA detection in genomic studies. As such, this step should be as analytically sensitive, efficient and reproducible as possible, especi...

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Главные авторы: John B A Okello, Linda Rodriguez, Debi Poinar, Kirsten Bos, Andrew L Okwi, Gabriel S Bimenya, Nelson K Sewankambo, Kenneth R Henry, Melanie Kuch, Hendrik N Poinar
Формат: Artigo
Язык:Inglês
Опубликовано: Public Library of Science (PLoS) 2010-11-01
Серии:PLoS ONE
Online-ссылка:http://europepmc.org/articles/PMC2978101?pdf=render
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