Quantitative assessment of the sensitivity of various commercial reverse transcriptases based on armored HIV RNA.

The in-vitro reverse transcription of RNA to its complementary DNA, catalyzed by the enzyme reverse transcriptase, is the most fundamental step in the quantitative RNA detection in genomic studies. As such, this step should be as analytically sensitive, efficient and reproducible as possible, especi...

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Autors principals: John B A Okello, Linda Rodriguez, Debi Poinar, Kirsten Bos, Andrew L Okwi, Gabriel S Bimenya, Nelson K Sewankambo, Kenneth R Henry, Melanie Kuch, Hendrik N Poinar
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science (PLoS) 2010-11-01
Col·lecció:PLoS ONE
Accés en línia:http://europepmc.org/articles/PMC2978101?pdf=render
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