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Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize

Abstract Prime editing is a novel and universal CRISPR/Cas-derived precision genome-editing technology that has been recently developed. However, low efficiency of prime editing has been shown in transgenic rice lines. We hypothesize that enhancing pegRNA expression could improve prime-editing effic...

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Detalhes bibliográficos
Main Authors: Yuan-Yuan Jiang, Yi-Ping Chai, Min-Hui Lu, Xiu-Li Han, Qiupeng Lin, Yu Zhang, Qiang Zhang, Yun Zhou, Xue-Chen Wang, Caixia Gao, Qi-Jun Chen
Formato: Artigo
Idioma:Inglês
Publicado em: BMC 2020-10-01
Colecção:Genome Biology
Assuntos:
PE2
ALS
Acesso em linha:http://link.springer.com/article/10.1186/s13059-020-02170-5
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