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Shoot-tips vitrification protocol for red chicory (Cichorium intybus L.) lines
Shoot tips from in vitro stock plants of red chicory ‘Rosso di Chioggia’ line were cryopreserved by one-step vitrification. After two days of cold-hardening on hormone-free MS medium and loading for 30 min in a mixture of 2 M glycerol and 0.4 M sucrose at 25°C, shoot tips were dehydrated with PVS2 v...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Firenze University Press
2013-05-01
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| Colecção: | Advances in Horticultural Science |
| Assuntos: | |
| Acesso em linha: | https://oaj.fupress.net/index.php/ahs/article/view/2939 |
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