A Simple and Universal System for Gene Manipulation in <named-content content-type="genus-species">Aspergillus fumigatus</named-content>: <italic toggle="yes">In Vitro</italic>-Assembled Cas9-Guide RNA Ribonucleoproteins Coupled with Microhomology Repair Templates

ABSTRACT CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 is a novel genome-editing system that has been successfully established in Aspergillus fumigatus. However, the current state of the technology relies heavily on DNA-based expression cassettes for delivering Cas9 and the...

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Autori principali:	Qusai Al Abdallah, Wenbo Ge, Jarrod R. Fortwendel
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology 2017-12-01
Serie:	mSphere
Soggetti:	Aspergillus fumigatus CRISPR-Cas9 gene deletion genome editing in vitro assembly pksP gene
Accesso online:	https://journals.asm.org/doi/10.1128/mSphere.00446-17
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