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DNA-Based Sensor for Real-Time Measurement of the Enzymatic Activity of Human Topoisomerase I

Sensors capable of quantitative real-time measurements may present the easiest and most accurate way to study enzyme activities. Here we present a novel DNA-based sensor for specific and quantitative real-time measurement of the enzymatic activity of the essential human enzyme, topoisomerase I. The...

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Autori principali: Birgitta Ruth Knudsen, Oskar Franch, Joanna Proszek, Yi-Ping Ho, Magnus Stougaard, Emil Laust Kristoffersen, Lærke Bay Marcussen, Morten Leth Jepsen
Natura: Artigo
Lingua:Inglês
Pubblicazione: MDPI AG 2013-03-01
Serie:Sensors
Soggetti:
real-time activity measurement
DNA sensor
fluorophore-quencher pair
topoisomerase I
cancer treatment
camptothecin
Accesso online:http://www.mdpi.com/1424-8220/13/4/4017
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