Imaging mitochondrial flux in single cells with a FRET sensor for pyruvate.

Mitochondrial flux is currently accessible at low resolution. Here we introduce a genetically-encoded FRET sensor for pyruvate, and methods for quantitative measurement of pyruvate transport, pyruvate production and mitochondrial pyruvate consumption in intact individual cells at high temporal resol...

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Hauptverfasser: Alejandro San Martín, Sebastián Ceballo, Felipe Baeza-Lehnert, Rodrigo Lerchundi, Rocío Valdebenito, Yasna Contreras-Baeza, Karin Alegría, L Felipe Barros
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science (PLoS) 2014-01-01
Schriftenreihe:PLoS ONE
Online Zugang:https://doi.org/10.1371/journal.pone.0085780
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