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Site-Directed Mutagenesis and Generation of Chimeric Viruses by Homologous Recombination in Yeast to Facilitate Analysis of Plant-Virus Interactions

A yeast homologous recombination system was used to generate mutants and chimeras in the genome of Potato leafroll virus (PLRV). A yeast-bacteria shuttle vector was developed that allows mutants and chimeras generated in yeast to be transformed into Escherichia coli for confirmation of the mutations...

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Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Delin Liang, Stewart M. Gray, Igor Kaplan, Peter Palukaitis
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: The American Phytopathological Society 2004-06-01
Saila:Molecular Plant-Microbe Interactions
Sarrera elektronikoa:https://apsjournals.apsnet.org/doi/10.1094/MPMI.2004.17.6.571
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