Site-Directed Mutagenesis and Generation of Chimeric Viruses by Homologous Recombination in Yeast to Facilitate Analysis of Plant-Virus Interactions
A yeast homologous recombination system was used to generate mutants and chimeras in the genome of Potato leafroll virus (PLRV). A yeast-bacteria shuttle vector was developed that allows mutants and chimeras generated in yeast to be transformed into Escherichia coli for confirmation of the mutations...
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| Autores principales: | , , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
The American Phytopathological Society
2004-06-01
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| Colección: | Molecular Plant-Microbe Interactions |
| Acceso en línea: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2004.17.6.571 |
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