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Cell passage number drives transcriptomic drift as an overlooked factor in experimental reproducibility

Abstract The reproducibility of scientific research has been increasingly challenged in recent years. While extrinsic factors (e.g., cross-contamination, mycoplasma infection, serum variability) are well-studied, the role of intrinsic attributes like cell passage number remains underexplored. Using...

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-д хадгалсан:
Номзүйн дэлгэрэнгүй
Үндсэн зохиолчид: Shuai Liu, Hailun Peng, Junyan Meng, Liyuan Zhuo, Shaoping Fu, Wei Yang
Формат: Artigo
Хэл сонгох:Inglês
Хэвлэсэн: Nature Portfolio 2025-11-01
Цуврал:Scientific Reports
Нөхцлүүд:
Онлайн хандалт:https://doi.org/10.1038/s41598-025-29424-1
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