Light-Controlled Affinity Purification of Protein Complexes Exemplified by the Resting ZAP70 Interactome

Multiprotein complexes control the behavior of cells, such as of lymphocytes of the immune system. Methods to affinity purify protein complexes and to determine their interactome by mass spectrometry are thus widely used. One drawback of these methods is the presence of false positives. In fact, the...

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Hlavní autoři:	Maximilian Hörner, Julian Eble, O. Sascha Yousefi, Jennifer Schwarz, Bettina Warscheid, Wilfried Weber, Wolfgang W. A. Schamel
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Frontiers Media S.A. 2019-02-01
Edice:	Frontiers in Immunology
Témata:	optogenetics affinity purification phytochrome ZAP70 protein-protein interaction mass spectrometry
On-line přístup:	https://www.frontiersin.org/article/10.3389/fimmu.2019.00226/full
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