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Light-Controlled Affinity Purification of Protein Complexes Exemplified by the Resting ZAP70 Interactome

Multiprotein complexes control the behavior of cells, such as of lymphocytes of the immune system. Methods to affinity purify protein complexes and to determine their interactome by mass spectrometry are thus widely used. One drawback of these methods is the presence of false positives. In fact, the...

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Principais autores: Maximilian Hörner, Julian Eble, O. Sascha Yousefi, Jennifer Schwarz, Bettina Warscheid, Wilfried Weber, Wolfgang W. A. Schamel
Format: Artigo
Jezik:Inglês
Izdano: Frontiers Media S.A. 2019-02-01
Serija:Frontiers in Immunology
Teme:
Online dostop:https://www.frontiersin.org/article/10.3389/fimmu.2019.00226/full
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