Identification of Biotinylated Molecules Using a Baculovirus-Expressed Luciferase-Streptavidin Fusion Protein
A genetic fusion between streptavidin of Streptomyces avidinii and luciferase of Pyrophorus plagiophthalamus was constructed. The fusion protein was produced in the Sf9 insect cell line using the baculovirus expression vector system (REVS). Sodium dodecyl sulfate polyacrylamide gel electrophoresis o...
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| Principais autores: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Taylor & Francis Group
1996-03-01
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| coleção: | BioTechniques |
| Acesso em linha: | https://www.future-science.com/doi/10.2144/19962003452 |
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