Identification of Biotinylated Molecules Using a Baculovirus-Expressed Luciferase-Streptavidin Fusion Protein
A genetic fusion between streptavidin of Streptomyces avidinii and luciferase of Pyrophorus plagiophthalamus was constructed. The fusion protein was produced in the Sf9 insect cell line using the baculovirus expression vector system (REVS). Sodium dodecyl sulfate polyacrylamide gel electrophoresis o...
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| Autores principales: | , , , , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Taylor & Francis Group
1996-03-01
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| Colección: | BioTechniques |
| Acceso en línea: | https://www.future-science.com/doi/10.2144/19962003452 |
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