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Identification of Biotinylated Molecules Using a Baculovirus-Expressed Luciferase-Streptavidin Fusion Protein

A genetic fusion between streptavidin of Streptomyces avidinii and luciferase of Pyrophorus plagiophthalamus was constructed. The fusion protein was produced in the Sf9 insect cell line using the baculovirus expression vector system (REVS). Sodium dodecyl sulfate polyacrylamide gel electrophoresis o...

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書誌詳細
主要な著者: M. Karp, C. Lindqvist, R. Nissinen, S. Wahlbeck, K. Åkerman, C. Oker-Blom
フォーマット: Artigo
言語:Inglês
出版事項: Taylor & Francis Group 1996-03-01
シリーズ:BioTechniques
オンライン・アクセス:https://www.future-science.com/doi/10.2144/19962003452
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