Monitoring Protein Splicing Using In-gel Fluorescence Immediately Following SDS-PAGE
Inteins garner significant interest from both basic and applied researchers due to their unique catalytic abilities. Herein, we describe a protocol for accurately monitoring protein splicing without purification using in-gel fluorescence immediately following Tris-Glycine SDS-PAGE. Following express...
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| Hlavní autoři: | , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Bio-protocol LLC
2021-08-01
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| Edice: | Bio-Protocol |
| On-line přístup: | https://bio-protocol.org/en/bpdetail?id=4121&type=0 |
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