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Monitoring Protein Splicing Using In-gel Fluorescence Immediately Following SDS-PAGE

Inteins garner significant interest from both basic and applied researchers due to their unique catalytic abilities. Herein, we describe a protocol for accurately monitoring protein splicing without purification using in-gel fluorescence immediately following Tris-Glycine SDS-PAGE. Following express...

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Sonraí bibleagrafaíochta
Príomhchruthaitheoirí: Joel Weinberger II, Christopher Lennon
Formáid: Artigo
Teanga:Inglês
Foilsithe / Cruthaithe: Bio-protocol LLC 2021-08-01
Sraith:Bio-Protocol
Rochtain ar líne:https://bio-protocol.org/en/bpdetail?id=4121&type=0
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