Kinetic characterization of amino acid activation by aminoacyl‐tRNA synthetases using radiolabelled γ‐[32P]ATP

Aminoacyl‐tRNA synthetases (AARSs) are fundamental enzymes that pair amino acids and tRNAs for protein synthesis. Aminoacylation occurs in two discrete steps. The amino acid is first activated by ATP, leading to an aminoacyl‐adenylate intermediate and pyrophosphate (PPi) formation. In a subsequent s...

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Үндсэн зохиолчид:	Igor Živković, Morana Dulic, Petra Kozulic, Marko Mocibob, Ita Gruic‐Sovulj
Формат:	Artigo
Хэл сонгох:	Inglês
Хэвлэсэн:	Wiley 2025-04-01
Цуврал:	FEBS Open Bio
Нөхцлүүд:	amino acid activation aminoacyl‐tRNA synthetases ATP/PPi exchange isotopic equilibrium exchange
Онлайн хандалт:	https://doi.org/10.1002/2211-5463.13903
Шошгууд:	Шошго нэмэх Шошго байхгүй, Энэхүү баримтыг шошголох эхний хүн болох!