Kinetic characterization of amino acid activation by aminoacyl‐tRNA synthetases using radiolabelled γ‐[32P]ATP

Aminoacyl‐tRNA synthetases (AARSs) are fundamental enzymes that pair amino acids and tRNAs for protein synthesis. Aminoacylation occurs in two discrete steps. The amino acid is first activated by ATP, leading to an aminoacyl‐adenylate intermediate and pyrophosphate (PPi) formation. In a subsequent s...

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Auteurs principaux:	Igor Živković, Morana Dulic, Petra Kozulic, Marko Mocibob, Ita Gruic‐Sovulj
Format:	Artigo
Langue:	Inglês
Publié:	Wiley 2025-04-01
Collection:	FEBS Open Bio
Sujets:	amino acid activation aminoacyl‐tRNA synthetases ATP/PPi exchange isotopic equilibrium exchange
Accès en ligne:	https://doi.org/10.1002/2211-5463.13903
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