Kinetic characterization of amino acid activation by aminoacyl‐tRNA synthetases using radiolabelled γ‐[32P]ATP

Aminoacyl‐tRNA synthetases (AARSs) are fundamental enzymes that pair amino acids and tRNAs for protein synthesis. Aminoacylation occurs in two discrete steps. The amino acid is first activated by ATP, leading to an aminoacyl‐adenylate intermediate and pyrophosphate (PPi) formation. In a subsequent s...

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Autors principals:	Igor Živković, Morana Dulic, Petra Kozulic, Marko Mocibob, Ita Gruic‐Sovulj
Format:	Artigo
Idioma:	Inglês
Publicat:	Wiley 2025-04-01
Col·lecció:	FEBS Open Bio
Matèries:	amino acid activation aminoacyl‐tRNA synthetases ATP/PPi exchange isotopic equilibrium exchange
Accés en línia:	https://doi.org/10.1002/2211-5463.13903
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