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Development of a Novel Method to Detect AAV Vector Integration

AAV integration has become an important safety consideration in gene therapy. However, accurately determining integration sites remains challenging due to biases introduced by library preparation methods, sequencing technologies, and bioinformatic pipelines. In this study, we developed a PCR-free am...

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Principais autores: Junping Zhang, Thao Thi Dang, Tsai-Yu Lin, Xiangping Yu, Danilo Pellin, Jiahe Tian, Olga Simmons, Emma Kou, Kenneth Cornetta, Weidong Xiao
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI AG 2026-03-01
coleção:Viruses
Assuntos:
AAV vector
AAV integration
CRISPR-Cas9
nanopore
sequencing
hybridization capture
Acesso em linha:https://www.mdpi.com/1999-4915/18/3/315
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