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Protocol for evaluating mitochondrial respiration in iPSC-derived neurons by the Seahorse XF analyzer

Summary: Here, we present a protocol describing the quantification of oxygen consumption rate (OCR) and maximal respiration rate (MRR) in living induced pluripotent stem cell (iPSC)-derived neurons using the Seahorse analyzer. We guide you through the whole process: culture amplification and seeding...

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Váldodahkkit: Chiara Fasano, Andrea Cavaliere, Valeria Tiranti, Camille Peron
Materiálatiipa: Artigo
Giella:Inglês
Almmustuhtton: Elsevier 2024-09-01
Ráidu:STAR Protocols
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Liŋkkat:http://www.sciencedirect.com/science/article/pii/S2666166724002922
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