Protocol to assess bioenergetics and mitochondrial fuel usage in murine autoreactive immunocytes using the Seahorse Extracellular Flux Analyzer

Summary: Efficient metabolism, or the means by which cells produce energy resources, is critical for proper effector function. Here, we present a protocol for examining the bioenergetics and mitochondrial fuel utilization of primary murine autoreactive immunocytes using cellular metabolism-modulatin...

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Autori principali:	Colleen L. Mayberry, John J. Wilson, Britney Sison, Chih-Hao Chang
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Elsevier 2024-06-01
Serie:	STAR Protocols
Soggetti:	Cell-based Assays Immunology Metabolism
Accesso online:	http://www.sciencedirect.com/science/article/pii/S2666166724001369
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