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Protocol for real-time measurement of mitochondrial respiration in the mouse ocular posterior pole using a Seahorse XFe24 analyzer

Summary: During aging and in retinal degenerative diseases, vulnerable retinal pigment epithelial (RPE) cells are subject to mitochondrial dysfunction, creating a need for accessibility to tools which can facilitate assessment of the ocular posterior pole bioenergetics. Here, we present a protocol f...

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Bibliografiske detaljer
Principais autores: Tanu Parmar, Vipul M. Parmar, Goldis Malek
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2024-09-01
Serier:STAR Protocols
Fag:
Online adgang:http://www.sciencedirect.com/science/article/pii/S2666166724003150
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