Engineer chimeric Cas9 to expand PAM recognition based on evolutionary information

The genomic locations that can be targeted for editing by CRISPR are limited by the presence of the nuclease-specific PAM sequence. Here, the authors show PAM recognition can be expanded by replacing the key region in the PAM interaction domain of SaCas9 with the corresponding region of SaCas9 ortho...

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Hlavní autoři:	Dacheng Ma, Zhimeng Xu, Zhaoyu Zhang, Xi Chen, Xiangzhi Zeng, Yiyang Zhang, Tingyue Deng, Mengfei Ren, Zheng Sun, Rui Jiang, Zhen Xie
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Nature Portfolio 2019-02-01
Edice:	Nature Communications
On-line přístup:	https://doi.org/10.1038/s41467-019-08395-8
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