Fully computational design of PAM-relaxed Staphylococcus aureus Cas9 with expanded targeting capability using UniDesign

CRISPR–Cas9 nucleases have transformed genome engineering, yet their application is often constrained by protospacer-adjacent motif (PAM) requirements. Staphylococcus aureus Cas9 (SaCas9) is particularly attractive for in vivo applications due to its compact size; however, its NNGRRT PAM limits targ...

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Detalhes bibliográficos

Principais autores:	Youcai Xiong, Li-Kuang Tsai, Jun Zhou, Shuang Chen, Xiaofeng Xia, Jifeng Zhang, Y Eugene Chen, Jie Xu, Xiaoqiang Huang
Formato:	Artigo
Idioma:	Inglês
Publicado em:	eLife Sciences Publications Ltd 2026-05-01
coleção:	eLife
Assuntos:	CRISPR-Cas9 SaCas9 PAM relaxation computational protein design genome editing base editing
Acesso em linha:	https://elifesciences.org/articles/110906
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