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Advanced Bioluminescence Reporter with Engineered <i>Gaussia</i> Luciferase via Sequence-Guided Mutagenesis

<i>Gaussia</i> luciferase (<i>G</i>Luc) is the preeminent secreted luciferase widely used in cell-based reporter assays. By employing sequence-guided mutagenesis informed by alignments of diverse copepod luciferase sequences, we identified key amino acids that significantly enhance bioluminescence (...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Vinayakumar Gedi, Eun Hye Kim, Bohyun Oh, Young-Pil Kim
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: MDPI AG 2024-11-01
Cyfres:Biosensors
Pynciau:
Mynediad Ar-lein:https://www.mdpi.com/2079-6374/14/11/528
Tagiau: Ychwanegu Tag
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