Advanced Bioluminescence Reporter with Engineered <i>Gaussia</i> Luciferase via Sequence-Guided Mutagenesis

<i>Gaussia</i> luciferase (<i>G</i>Luc) is the preeminent secreted luciferase widely used in cell-based reporter assays. By employing sequence-guided mutagenesis informed by alignments of diverse copepod luciferase sequences, we identified key amino acids that significantly enhance bioluminescence (...

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Detalhes bibliográficos

Principais autores:	Vinayakumar Gedi, Eun Hye Kim, Bohyun Oh, Young-Pil Kim
Formato:	Artigo
Idioma:	Inglês
Publicado em:	MDPI AG 2024-11-01
Colecção:	Biosensors
Assuntos:	bioluminescence <i>Gaussia</i> luciferase mutagenesis coelenterazine reporter
Acesso em linha:	https://www.mdpi.com/2079-6374/14/11/528
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