Advanced Bioluminescence Reporter with Engineered <i>Gaussia</i> Luciferase via Sequence-Guided Mutagenesis
<i>Gaussia</i> luciferase (<i>G</i>Luc) is the preeminent secreted luciferase widely used in cell-based reporter assays. By employing sequence-guided mutagenesis informed by alignments of diverse copepod luciferase sequences, we identified key amino acids that significantly enhance bioluminescence (...
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| Główni autorzy: | , , , |
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| Format: | Artigo |
| Język: | Inglês |
| Wydane: |
MDPI AG
2024-11-01
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| Seria: | Biosensors |
| Hasła przedmiotowe: | |
| Dostęp online: | https://www.mdpi.com/2079-6374/14/11/528 |
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