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Allele-Specific PCR for PIK3CA Mutation Detection Using Phosphoryl Guanidine Modified Primers

Phosphoryl guanidine (PG) is the novel uncharged modification of internucleotide phosphates of oligonucleotides. Incorporating PG modification into PCR primers leads to increased discrimination between wild-type and mutated DNA, providing extraordinary detection limits in an allele-specific real-tim...

Whakaahuatanga katoa

I tiakina i:
Ngā taipitopito rārangi puna kōrero
Ngā kaituhi matua: Alexey S. Chubarov, Igor P. Oscorbin, Lidiya M. Novikova, Maxim L. Filipenko, Alexander A. Lomzov, Dmitrii V. Pyshnyi
Hōputu: Artigo
Reo:Inglês
I whakaputaina: MDPI AG 2023-01-01
Rangatū:Diagnostics
Ngā marau:
Urunga tuihono:https://www.mdpi.com/2075-4418/13/2/250
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