Multi-gene precision editing tool using CRISPR-Cas12a/Cpf1 system in Ogataea polymorpha

Abstract Background Ogataea polymorpha, a non-conventional methylotrophic yeast, has demonstrated significant potential for heterologous protein expression and the production of high-value chemicals and biopharmaceuticals. However, the lack of precise and efficient genome editing tools severely hind...

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主要な著者:	Senqin Hou, Shibin Yang, Wenqin Bai
フォーマット:	Artigo
言語:	Inglês
出版事項:	BMC 2025-01-01
シリーズ:	Microbial Cell Factories
主題:	Ogataea polymorpha CRISPR-Cpf1 Homologous recombination rates Large DNA fragment deletion Multiple genes integration
オンライン･アクセス:	https://doi.org/10.1186/s12934-025-02654-8
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